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辅酶 Q10 在他克莫司诱导的β细胞损伤过程中对线粒体功能障碍的治疗潜力

罗 康, 罗 康, 朴 林虎, 林 善宇, 梁 哲宇

摘要

我们先前报道过,长期他克莫司治疗引起的氧化应激会损害胰腺β细胞的线粒体功能。本研究旨在探讨辅酶Q10(Coenzyme Q10)在他克莫司诱导的糖尿病大鼠模型中改善线粒体功能障碍的治疗潜力。在大鼠他克莫司诱导的糖尿病模型中,(Coenzyme Q10)CoQ10治疗改善了胰腺β细胞功能;CoQ10 可增强胰岛内的胰岛素免疫反应性,同时伴随氧化应激和细胞凋亡的减轻。通过电子显微镜对线粒体超微结构的评估显示,与单独使用他克莫司治疗相比,CoQ10 治疗显著增加了线粒体的大小、数量和体积,以及胰岛素颗粒的数量。一项使用胰腺β细胞系的体外研究表明,他克莫司治疗会增加细胞凋亡及线粒体活性氧的产生,而与 CoQ10 联合治疗则能有效减轻这些变化。在亚细胞水平上,CoQ10 显著改善了他克莫司引起的线粒体呼吸功能损伤,具体表现为线粒体耗氧量和 ATP 产量的增加。我们的数据表明,CoQ10 在减轻他克莫司诱导的氧化应激及保护胰腺β细胞线粒体方面发挥重要作用。这些结果提示,补充 CoQ10 对他克莫司诱发的糖尿病具有有益作用。

关键词

他克莫司;线粒体功能

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参考

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