吲哚菁绿显影小鼠胆管并益于左外叶门静脉的结扎
摘要
通过静脉注射吲哚菁绿溶液，并同时结扎胆总管，数分钟后可使小鼠（Mus musculus）肝外胆道系统呈翠绿色，可见各肝
叶胆管常于近胆囊处汇入胆总管。吲哚菁绿肝外胆管造影后，利用 25 × 显微镜观察小鼠左外叶肝脏脉管系统，将小鼠左
外叶肝脏分为隐藏型、显露差型、显露一般型和显露完全型。选择显露一般型和显露完全型，用 10-0 带线缝合针于左外
叶脏面，包绕左外叶门静脉进针和出针，外科结结扎。手术后 3 天观察，左外叶肝脏鲜活，未结扎叶肝脏 Ki67 阳性细胞核
占比提高，肝叶重量明显增加，成功诱导了肝脏再生。
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